Illumina, Inc. v. Ariosa Diagnostics, Inc.

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Case: 19-1419 Document: 55 Page: 1 Filed: 03/17/2020 United States Court of Appeals for the Federal Circuit ______________________ ILLUMINA, INC., SEQUENOM, INC., Plaintiffs-Appellants v. ARIOSA DIAGNOSTICS, INC., ROCHE SEQUENCING SOLUTIONS, INC., ROCHE MOLECULAR SYSTEMS, INC., Defendants-Appellees ______________________ 2019-1419 ______________________ Appeal from the United States District Court for the Northern District of California in No. 3:18-cv-02847-SI, Senior Judge Susan Y. Illston. ______________________ Decided: March 17, 2020 ______________________ EDWARD R. REINES, Weil, Gotshal & Manges LLP, Red- wood Shores, CA, argued for plaintiffs-appellants. Also represented by CHRISTOPHER SHAWN LAVIN, DEREK C. WALTER. DARALYN JEANNINE DURIE, Durie Tangri LLP, San Francisco, CA, argued for all defendants-appellees. De- fendant-appellee Ariosa Diagnostics, Inc. also represented by DAVID FLOYD MCGOWAN, LAURA MILLER. Case: 19-1419 Document: 55 Page: 2 Filed: 03/17/2020 2 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. ROBERT J. GUNTHER, JR., Wilmer Cutler Pickering Hale and Dorr LLP, New York, NY, for defendants-appellees Roche Sequencing Solutions, Inc., Roche Molecular Sys- tems, Inc. Also represented by OMAR KHAN, CHRISTOPHER R. NOYES; THOMAS SAUNDERS, Washington, DC. ______________________ Before LOURIE, MOORE, and REYNA, Circuit Judges. Opinion for the court filed by Circuit Judge LOURIE. Dissenting opinion filed by Circuit Judge REYNA. LOURIE, Circuit Judge. Illumina, Inc. and Sequenom, Inc. (collectively, “Illu- mina”) appeal from a decision of the United States District Court for the Northern District of California that claims 1– 2, 4–5, and 9–10 of U.S. Patent 9,580,751 (the “’751 pa- tent”) and claims 1–2 and 10–14 of U.S. Patent 9,738,931 (the “’931 patent”) are invalid under 35 U.S.C. § 101 as di- rected to an ineligible natural phenomenon. Illumina, Inc. v. Ariosa Diagnostics, Inc., 356 F. Supp. 3d 925 (N.D. Cal. 2018) (“Decision”). Because we conclude that the claims are directed to patent-eligible subject matter, we reverse. BACKGROUND “In 1996, Drs. Dennis Lo and James Wainscoat discov- ered cell-free fetal DNA in maternal plasma and serum, the portion of maternal blood samples that other researchers had previously discarded as medical waste.” Ariosa Diag- nostics, Inc. v. Sequenom, Inc., 788 F.3d 1371, 1373 (Fed. Cir. 2015). They applied for a patent, and, in 2001, they obtained U.S. Patent 6,258,540, which claimed a method for detecting the small fraction of paternally inherited cell- free fetal DNA in the plasma and serum of a pregnant woman. Id. In 2015, we held that the claims of that patent were invalid under 35 U.S.C. § 101 because they were di- rected to “matter that is naturally occurring”—i.e., the Case: 19-1419 Document: 55 Page: 3 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 3 natural phenomenon that cell-free fetal DNA exists in ma- ternal blood. Id. at 1376. The present case involves two patents that are unre- lated to the patent held invalid in Ariosa, but rather claim priority from a European patent application filed in 2003. The ’751 and ’931 patents at issue in this case, which are related to each other and have largely identical specifica- tions, begin by acknowledging the natural phenomenon that was at issue in Ariosa: “[I]t has been shown that in the case of a pregnant woman extracellular fetal DNA is pre- sent in the maternal circulation and can be detected in ma- ternal plasma . . . .” ’751 patent col. 1 ll. 23–25. The patents then identify a problem that was the subject of fur- ther research on cell-free fetal DNA in maternal blood: [T]he major proportion (generally >90%) of the ex- tracellular DNA in the maternal circulation is de- rived from the mother. This vast bulk of maternal circulatory extracellular DNA renders it difficult, if not impossible, to determine fetal genetic alterna- tions [sic] . . . from the small amount of circulatory extracellular fetal DNA. Id. col. 1 ll. 42–50. In simple terms, the problem that the inventors encountered was that, although it was known that cell-free fetal DNA existed in the mother’s blood- stream, there was no known way to distinguish and sepa- rate the tiny amount of fetal DNA from the vast amount of maternal DNA. The inventors of the ’751 and ’931 patents attempted to find a solution to that problem. First, they made a discov- ery: An examination of circulatory extracellular fetal DNA and circulatory extracellular maternal DNA in maternal plasma has now shown that, surpris- ingly, the majority of the circulatory extracellular fetal DNA has a relatively small size of Case: 19-1419 Document: 55 Page: 4 Filed: 03/17/2020 4 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. approximately 500 base pairs or less, whereas the majority of circulatory extracellular maternal DNA in maternal plasma has a size greater than approx- imately 500 base pairs. Id. col. 1 ll. 54–61. Having made that discovery, they used it to develop a solution to the identified problem of distin- guishing fetal DNA from maternal DNA in the mother’s bloodstream: This surprising finding forms the basis of the pre- sent invention according to which separation of cir- culatory extracellular DNA fragments which are smaller than approximately 500 base pairs pro- vides a possibility to enrich for fetal DNA se- quences from the vast bulk of circulatory extracellular maternal DNA. Id. col. 2 ll. 1–6. The claims of the ’751 and ’931 patents are directed to that solution. Specifically, they claim methods of prepar- ing a fraction of cell-free DNA that is enriched in fetal DNA. Claim 1 is the only independent claim in each pa- tent: 1. A method for preparing a deoxyribonucleic acid (DNA) fraction from a pregnant human female useful for analyzing a genetic locus involved in a fetal chromosomal aberration, comprising: (a) extracting DNA from a substantially cell-free sample of blood plasma or blood serum of a preg- nant human female to obtain extracellular circula- tory fetal and maternal DNA fragments; (b) producing a fraction of the DNA extracted in (a) by: (i) size discrimination of extracellular circula- tory DNA fragments, and Case: 19-1419 Document: 55 Page: 5 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 5 (ii) selectively removing the DNA fragments greater than approximately 500 base pairs, wherein the DNA fraction after (b) comprises a plurality of genetic loci of the extracellular circula- tory fetal and maternal DNA; and (c) analyzing a genetic locus in the fraction of DNA produced in (b). ’751 patent col. 7 l. 54–col. 8 l. 57. 1. A method, comprising: (a) extracting DNA comprising maternal and fe- tal DNA fragments from a substantially cell-free sample of blood plasma or blood serum of a preg- nant human female; (b) producing a fraction of the DNA extracted in (a) by: (i) size discrimination of extracellular circula- tory fetal and maternal DNA fragments, and (ii) selectively removing the DNA fragments greater than approximately 300 base pairs, wherein the DNA fraction after (b) comprises ex- tracellular circulatory fetal and maternal DNA fragments of approximately 300 base pairs and less and a plurality of genetic loci of the extracellular circulatory fetal and maternal DNA fragments; and (c) analyzing DNA fragments in the fraction of DNA produced in (b). ’931 patent col. 7 l. 58–col. 8 l. 63. Dependent claims in each patent place further limita- tions on the size discrimination and selective removal pro- cesses recited in step (b) of the method claims. For example, dependent claim 7 of the ’751 patent recites that “the size discrimination in (b) comprises centrifugation,” Case: 19-1419 Document: 55 Page: 6 Filed: 03/17/2020 6 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. and claim 8 further limits it to “density gradient centrifu- gation.” ’751 patent col. 9 ll. 1–4. Likewise, dependent claims 4–10 of the ’931 patent recite that step (b) can com- prise “chromatography,” “electrophoresis,” “centrifuga- tion,” and/or “nanotechnological means.” ’931 patent col. 9 ll. 1–14. Illumina filed suit against Ariosa Diagnostics, Inc., Roche Sequencing Solutions, Inc., and Roche Molecular Systems, Inc. (collectively, “Roche”) alleging infringement of the ’751 and ’931 patents. Roche moved for summary judgment that the asserted claims are invalid under 35 U.S.C. § 101. The district court granted Roche’s motion for summary judgment, holding that the claims of the ’751 and ’931 patents are directed to ineligible subject matter. De- cision, 356 F. Supp. 3d at 935. The court entered judgment in favor of Roche, and Illumina appealed. We have juris- diction under 28 U.S.C. § 1295(a)(1). DISCUSSION We review a grant of summary judgment according to the law of the regional circuit. Kaneka Corp. v. Xiamen Kingdomway Grp. Co., 790 F.3d 1298, 1303 (Fed. Cir. 2015) (citing Halo Elecs., Inc. v. Pulse Elecs., Inc., 769 F.3d 1371, 1377 (Fed. Cir. 2014)). In the Ninth Circuit, a grant of summary judgment is reviewed de novo. Leever v. Carson City, 360 F.3d 1014, 1017 (9th Cir. 2004) (citing Hargis v. Foster, 312 F.3d 404, 409 (9th Cir. 2002)). Summary judg- ment is appropriate when “there is no genuine dispute as to any material fact and the movant is entitled to judgment as a matter of law.” Fed. R. Civ. P. 56. I Section 101 provides that “Whoever invents or discov- ers any new and useful process, machine, manufacture, or composition of matter, or any new and useful improvement thereof, may obtain a patent therefor . . . .” 35 U.S.C. § 101. Given the expansive terms of § 101, “Congress plainly Case: 19-1419 Document: 55 Page: 7 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 7 contemplated that the patent laws would be given wide scope”; the legislative history likewise indicated that “Con- gress intended statutory subject matter to ‘include any- thing under the sun that is made by man.’” Diamond v. Chakrabarty, 447 U.S. 303, 308–09 (1980) (internal cita- tion omitted). The Supreme Court has held that § 101 “contains an important implicit exception. ‘[L]aws of nature, natural phenomena, and abstract ideas’ are not patentable.” Mayo Collaborative Servs. v. Prometheus Labs., Inc., 566 U.S. 66, 70 (2012) (alteration in original) (quoting Diamond v. Diehr, 450 U.S. 175, 185 (1981)). These exceptions exist because monopolizing the basic tools of scientific work “might tend to impede innovation more than it would tend to promote it.” Id. at 71. However, the Supreme Court has advised that these exceptions must be applied cautiously, as “too broad an interpretation of this exclusionary princi- ple could eviscerate patent law.” Id. Laws of nature and natural phenomena are not patent- able, but applications and uses of such laws and phenom- ena may be patentable. A claim to otherwise statutory subject matter does not become ineligible by its use of a law of nature or natural phenomenon. See Diehr, 450 U.S. at 187; Parker v. Flook, 437 U.S. 584, 590 (1978). On the other hand, adding “conventional steps, specified at a high level of generality,” to a law of nature or natural phenomenon does not make a claim to the law or phenomenon patenta- ble. Mayo, 566 U.S. at 82. To distinguish claims to patent-eligible applications of laws of nature and natural phenomena from claims that impermissibly tie up such laws and phenomena, we apply the two-part test set forth by the Supreme Court. First, we examine whether the claims are “directed to” a law of na- ture or natural phenomenon. Alice Corp. Pty. Ltd. v. CLS Bank Int’l, 573 U.S. 208, 217 (2014). If—and only if—they are, then we proceed to the second inquiry, where we Case: 19-1419 Document: 55 Page: 8 Filed: 03/17/2020 8 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. examine whether the limitations of the claim apart from the law of nature or natural phenomenon, considered indi- vidually and as an ordered combination, “‘transform the nature of the claim’ into a patent-eligible application.” Id. (quoting Mayo, 566 U.S. at 78). II This is not a diagnostic case. And it is not a method of treatment case. It is a method of preparation case. Under Mayo, we have consistently held diagnostic claims unpatentable as directed to ineligible subject mat- ter. See Athena Diagnostics, Inc. v. Mayo Collaborative Servs., LLC, 927 F.3d 1333, 1352 (Fed. Cir. 2019) (Moore, J., dissenting from denial of rehearing en banc) (“Since Mayo, we have held every single diagnostic claim in every case before us ineligible.”); see also, e.g., Athena Diagnos- tics, Inc. v. Mayo Collaborative Servs., LLC, 915 F.3d 743 (Fed. Cir. 2019); Cleveland Clinic Found. v. True Health Diagnostics LLC, 859 F.3d 1352 (Fed. Cir. 2017); Cleveland Clinic Found. v. True Health Diagnostics LLC, 760 F. App’x 1013 (Fed. Cir. 2019). In contrast, we have held that method of treatment claims are patent-eligible. See Endo Pharm. Inc. v. Teva Pharm. USA, Inc., 919 F.3d 1347 (Fed. Cir. 2019); Natural Alternatives Int'l, Inc. v. Creative Com- pounds, LLC, 918 F.3d 1338 (Fed. Cir. 2019); Vanda Pharm. Inc. v. West-Ward Pharm. Int’l Ltd., 887 F.3d. 1117 (Fed. Cir. 2018). The claims in this case do not fall into either bucket, and we consider the claims under the Al- ice/Mayo test. Here, it is undisputed that the inventors of the ’751 and ’931 patents discovered a natural phenomenon. But at step one of the Alice/Mayo test, “it is not enough to merely iden- tify a patent-ineligible concept underlying the claim; we must determine whether that patent-ineligible concept is what the claim is ‘directed to.’” Rapid Litig. Mgmt. Ltd. v. CellzDirect, Inc., 827 F.3d 1042, 1050 (Fed. Cir. 2016). The focus of the dispute in this case is whether the claims of the Case: 19-1419 Document: 55 Page: 9 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 9 ’751 and ’931 patents are “directed to” the natural phenom- enon, i.e., whether they claim the discovered natural phe- nomenon itself versus eligible subject matter that exploits the discovery of the natural phenomenon. As an initial matter, there are differences between the district court and the parties about how to articulate the natural phenomenon that the inventors discovered. The district court appeared to find that the relevant natural phenomenon is either the “testable quantity” of fetal DNA or “test results” obtained from that fetal DNA. Decision, 356 F. Supp. 3d at 933. Roche’s articulation of the natural phenomenon was a moving target throughout its briefing and at oral argument, but appears to be the “size distribu- tion” of fetal to maternal cell-free DNA in a mother’s blood reflected in Table 1 of the specification, with a particular focus on the number “500 base pairs” as the critical divid- ing line between the two. See Appellee’s Br. 14, 18, 21; Oral Arg. 27:58, 28:35, 29:16. And Illumina asserts more simply that the inventors’ discovery was that “fetal cell-free DNA tends to be shorter than maternal cell-free DNA.” Appel- lant’s Br. 24; see also id. at 8 (“[I]n a sample of cell-free DNA from a pregnant woman, the DNA that arises from the fetus is smaller on average than the DNA that arises from the mother.”). We take note of Roche’s inability—despite its status as the party challenging the validity of the patents—to clearly identify the natural phenomenon that forms the ba- sis of its challenge. But, ultimately, we find that the par- ties’ respective articulations reflect distinctions without differences. For simplicity, we adopt Illumina’s articula- tion of the natural phenomenon, i.e., that cell-free fetal DNA tends to be shorter than cell-free maternal DNA in a mother’s bloodstream. We thus turn to the crucial question on which this case depends: whether the claims are “di- rected to” that natural phenomenon. We conclude that the claims are not directed to that natural phenomenon but ra- ther to a patent-eligible method that utilizes it. Case: 19-1419 Document: 55 Page: 10 Filed: 03/17/2020 10 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. The claims in this case are directed to methods for pre- paring a fraction of cell-free DNA that is enriched in fetal DNA. The methods include specific process steps—size dis- criminating and selectively removing DNA fragments that are above a specified size threshold—to increase the rela- tive amount of fetal DNA as compared to maternal DNA in the sample. ’751 patent col. 7 ll. 63–67. Those process steps change the composition of the mixture, resulting in a DNA fraction that is different from the naturally-occurring fraction in the mother’s blood. Thus, the process achieves more than simply observing that fetal DNA is shorter than maternal DNA or detecting the presence of that phenome- non. The dependent claims further illustrate the concrete nature of the claimed process steps. For example, claims 7–8 of the ’751 and claims 8–9 of the ’931 patent require that the size discrimination step comprise “centrifugation,” and specifically “density gradient centrifugation.” ’751 pa- tent col. 9 ll. 1–4; ’931 patent col. 9 ll. 9–12. Other depend- ent claims in the ’931 patent comprise other discrimination and separation means, such as “high performance liquid chromatography” (claims 4–5), “capillary electrophoresis” (claims 6–7), or “nanotechnological means” (claim 10). These dependent claims are supported by the specifica- tion’s description of the physical means by which the size discrimination and selective removal step of the claims can be achieved: The size separation of the extracellular DNA in said serum or plasma sample can be brought about by a variety of methods, including but not limited to: chromatography or electrophoresis such as chro- matography on agarose or polyacrylamide gels, ion- pair reversed-phase high performance liquid chro- matography [], capillary electrophoresis in a self- coating, low-viscosity polymer matrix [], selective extraction in microfabricated electrophoresis de- vices [], microchip electrophoresis on reduced Case: 19-1419 Document: 55 Page: 11 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 11 viscosity polymer matrices [], adsorptive mem- brane chromatography [] and the like; density gra- dient centrifugation []; and methods utilising [sic] nanotechnological means such as microfabricated entropic trap arrays [] and the like. ’931 patent col. 2 l. 61–col. 3 l. 18 (citations omitted); see also id. col. 4 ll. 15–22 (“3. The gel was electrophoresed at 80 Volt for 1 hour. 4. The Gel [sic] was cut into pieces cor- responding to specific DNA sizes . . . .”). As described by the specification, the inventors used these concrete process steps, not merely to observe the presence of the phenome- non that fetal DNA is shorter than maternal DNA, but ra- ther to exploit that discovery in a method for preparation of a mixture enriched in fetal DNA. Roche insists that the claims in this case are no more eligible than the claims at issue in Ariosa. We disagree. In Ariosa, the relevant independent claims were directed to a method “for detecting a paternally inherited nucleic acid” (claims 1 and 24) or a method “for performing a prenatal diagnosis” (claim 25). See Ariosa, 788 F.3d at 1373–74. The only operative steps in the claims were “amplifying” (i.e., making more of) the cell-free fetal DNA and then “de- tecting [it],” “subjecting [it] . . . to a test,” or “performing nucleic acid analysis on [it] to detect [it].” Id. We found those claims ineligible because, like the invalid diagnostic claims at issue in Mayo, Athena, and Cleveland Clinic, they were directed to detecting a natural phenomenon. In es- sence, the inventors in Ariosa discovered that cell-free fetal DNA exists, and then obtained patent claims that covered only the knowledge that it exists and a method to see that it exists. Here, in contrast, the claims are directed to more than just the correlation between a DNA fragment’s size and its tendency to be either fetal or maternal. And the claims do not merely cover a method for detecting whether a cell-free DNA fragment is fetal or maternal based on its size. Rather the claimed method removes some maternal DNA from the mother’s blood to prepare a fraction of cell- Case: 19-1419 Document: 55 Page: 12 Filed: 03/17/2020 12 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. free DNA that is enriched in fetal DNA. Thus, the claims in this case are different from the claims that we held in- valid in Ariosa. Roche also argues, based on the Supreme Court’s deci- sion in Association for Molecular Pathology v. Myriad Ge- netics, Inc., that “a naturally occurring DNA segment is a product of nature and not patent eligible merely because it has been isolated.” 569 U.S. 576, 580 (2013). But the claims here are not directed to the cell-free fetal DNA itself. The Supreme Court in Myriad expressly declined to extend its holding to method claims reciting a process used to iso- late DNA. See id. at 595–96. The Court stated: It is important to note what is not implicated by this decision. First, there are no method claims be- fore this Court. Had Myriad created an innovative method of manipulating genes while searching for the BRCA1 and BRCA2 genes, it could possibly have sought a method patent. But the processes used by Myriad to isolate DNA . . . are not at issue in this case. Id. Thus, in Myriad, the claims were ineligible because they covered a gene rather than a process for isolating it. Here, we encounter the opposite situation, i.e., the claims do not cover cell-free fetal DNA itself but rather a process for selective removal of non-fetal DNA to enrich a mixture in fetal DNA. Thus, the Supreme Court’s decision in Myr- iad is not on point. In our view, CellzDirect, while not directly on point, is instructive. In CellzDirect, the inventors discovered the natural phenomenon “that some fraction of hepatocytes are capable of surviving multiple freeze-thaw cycles.” 827 F.3d at 1045. Having made that discovery, they patented an “improved process of preserving hepatocytes,” that com- prises freezing hepatocytes, thawing the hepatocytes, re- moving the non-viable hepatocytes, and refreezing the viable hepatocytes. Id. We found that their claimed Case: 19-1419 Document: 55 Page: 13 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 13 invention was patent-eligible because it was “not simply an observation or detection of the ability of hepatocytes to sur- vive multiple freeze-thaw cycles. Rather, the claims are directed to a new and useful method of preserving hepato- cyte cells.” Id. at 1048. The inventors in CellzDirect did not invent hepatocytes or impart to hepatocytes an ability to survive cycles of freezing and thawing. Id. at 1045. Rather, they discovered that hepatocytes naturally have that ability, and they ex- ploited that phenomenon in a patent-eligible method. So too here, the inventors of the ’751 and ’931 patents obvi- ously did not invent cell-free fetal DNA or the relative size distribution of fetal and maternal cell-free DNA in mater- nal blood. And, like in CellzDirect, the inventors used their discovery to invent a method of preparing a fraction of DNA that includes physical process steps to selectively remove some maternal DNA in blood to produce a mixture enriched in fetal DNA. Roche argues that the techniques for size discriminat- ing and selectively removing DNA fragments that are used to practice the invention were well-known and conven- tional. And we recognize, of course, that the inventors of the ’751 and ’931 patents did not invent centrifugation, chromatography, electrophoresis, or nanotechnology. 1 But while such considerations may be relevant to the inquiry under Alice/Mayo step two, or to other statutory considera- tions such as obviousness that are not at issue before us in this case, they do not impact the Alice/Mayo step one ques- tion whether the claims themselves are directed to a natu- ral phenomenon. Again, CellzDirect is instructive, where we acknowledged that the inventors had not invented the 1 We note, without deciding, that Illumina argues that claim 11 of the ’931 patent requires the use of micro- arrays, which it claims was a methodology not previously used with cell-free DNA. Appellant’s Br. 40. Case: 19-1419 Document: 55 Page: 14 Filed: 03/17/2020 14 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. well-known processes of “freezing” and “thawing,” but only in the context of the Alice/Mayo step two inquiry. 827 F.3d at 1050–51. Rather than focusing on what the inventors of the ’751 and ’931 patents did not invent, we focus our Alice/Mayo step one analysis on what the inventors did purport to in- vent and what they claimed in their patents: methods for preparing a fraction of cell-free DNA by the physical pro- cess of size discriminating and selectively removing DNA fragments longer than a specified threshold. Those meth- ods are “directed to” more than merely the natural phe- nomenon that the inventors discovered. Accordingly, we conclude at step one of the Alice/Mayo test that the claims are not directed to a patent-ineligible concept, and we need not reach step two of the test. III In Ariosa, we recognized that the inventors had made a discovery with implications that would allow what had previously been discarded as medical waste to be used as a tool for determining fetal characteristics. 788 F.3d at 1373. We acknowledged the profound impact that the discovery had on the field of prenatal medicine, including that it “cre- ated an alternative for prenatal diagnosis of fetal DNA that avoids the risks of widely-used techniques that took sam- ples from the fetus or placenta.” Id. Nevertheless, under guidance from the Supreme Court, we determined that the discovery of that natural phenomenon, no matter how sig- nificant it was to the medical field, was not itself patenta- ble, and neither was a method for detecting it. Id. at 1379– 80. The invention in this case is the product of further re- search on cell-free fetal DNA. This time, the inventors dis- covered that, not only does the fetal DNA exist in the bloodstream of a pregnant mother, but it has characteris- tics that make it distinguishable, and therefore separable, from the maternal DNA. Again, regardless how Case: 19-1419 Document: 55 Page: 15 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 15 groundbreaking this additional discovery may have been, the inventors were not entitled to patent the natural phe- nomenon that cell-free fetal DNA tends to be shorter than cell-free maternal DNA. “Groundbreaking, innovative, or even brilliant discovery does not by itself satisfy the § 101 inquiry.” Myriad, 569 U.S. at 591. Thus, they could not claim a method directed to the natural phenomenon, e.g., a method for determining whether a fragment of cell-free DNA is fetal or maternal based on its length. And they did not attempt to patent such a method. The inventors here patented methods of preparing a DNA fraction. The claimed methods utilize the natural phenomenon that the inventors discovered by employing physical process steps to selectively remove larger frag- ments of cell-free DNA and thus enrich a mixture in cell- free fetal DNA. Though we make no comment on whether the claims at issue will pass muster under challenges based on any other portion of the patent statute, under § 101 the claimed methods are patent-eligible subject matter. CONCLUSION We conclude that the claims of the ’751 and ’931 pa- tents are directed to patent-eligible subject matter under 35 U.S.C. § 101. We therefore reverse the district court’s grant of summary judgment and remand for further pro- ceedings. REVERSED AND REMANDED Case: 19-1419 Document: 55 Page: 16 Filed: 03/17/2020 United States Court of Appeals for the Federal Circuit ______________________ ILLUMINA, INC., SEQUENOM, INC., Plaintiffs-Appellants v. ARIOSA DIAGNOSTICS, INC., ROCHE SEQUENCING SOLUTIONS, INC., ROCHE MOLECULAR SYSTEMS, INC., Defendants-Appellees ______________________ 2019-1419 ______________________ Appeal from the United States District Court for the Northern District of California in No. 3:18-cv-02847-SI, Senior Judge Susan Y. Illston. ______________________ REYNA, Circuit Judge, dissenting. The Majority holds that the asserted patents are di- rected to patent-eligible subject matter. I respectfully dis- agree and dissent. I conclude that the claims are directed to a natural phenomenon. The patents’ only claimed ad- vance is the discovery of that natural phenomenon. The claims, the written description, and the legal precedent ap- plicable to this case all support the conclusion that the pa- tents are ineligible. Case: 19-1419 Document: 55 Page: 17 Filed: 03/17/2020 2 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. I. The ’751 and ’931 Patents 1 At the time of the invention, skilled artisans knew that cell-free fetal DNA (“cff-DNA”) existed, that it could be de- tected in a sample of a pregnant woman’s blood or serum, and that it was useful for reliably analyzing fetal genetic markers (for detecting certain diseases and disorders). ’751 patent col. 1 ll. 22–34. But for some genetic markers that are found in the genomes of both the mother and the fetus, skilled artisans faced a problem: the relatively small amount of cff-DNA compared to maternal extracellular DNA in the mother’s blood made it difficult to identify and analyze genetic alterations in the fetus. Id. at col. 1 ll. 41– 50. The patent maintains that the problem was overcome when the inventors made a “surprising” discovery. Id. at col. 1 ll. 54–61. The inventors discovered a natural phe- nomenon: that cff-DNA tends to be shorter than cell-free maternal DNA in a mother’s blood. See id. at col. 1 ll. 54– 67; see also Maj. Op. at 3–4, 8. The written description ex- plains that the majority of cff-DNA in the mother’s blood “has a relatively small size of approximately 500 base pairs or less, whereas the majority of circulatory extracellular maternal DNA in maternal plasma has a size greater than approximately 500 base pairs.” Id. at col. 1 ll. 54–61. The written description states that “[t]his surprising finding forms the basis of the present invention.” Id. at col. 2 ll. 1– 2 (emphasis added). Other than the surprising discovery, nothing else in the specification or the record before us indicates there was anything new or useful about the claimed invention. In two 1 U.S. Patent Nos. 9,580,751 and 9,738,931. The pa- tents contain nearly identical written descriptions and claims. For economy, this opinion will reference only the ’751 patent. Case: 19-1419 Document: 55 Page: 18 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 3 examples, the patent describes experiments that illustrate the natural phenomenon and a potential application. Id. at col. 3 l. 30–col. 6 l. 46. The results of Example 1, as cap- tured in Table 1, demonstrate that “DNA fragments origi- nating from the fetus were almost completely of sizes smaller than 500 base pairs with around 70% being of fetal origin for sizes smaller than 300 bases.” Id. at col. 4 l. 50– col. 5 l. 7. The results of Example 2 demonstrate that fetal alleles for “D21S11,” a genetic marker found in the human chromosome related to Down Syndrome, could be detected in cell-free DNA samples from which fragments greater than 500 base pairs or 300 base pairs had been removed. Both experiments were conducted using known laboratory techniques and commercially available testing kits. E.g., id. at col. 3 ll. 49–50, col. 3 l. 65–col. 4 l. 13, col. 5 ll. 45–50; see also id. at col. 2 l. 61–col. 3 l. 18. The claims recite nearly identical method steps. The method steps of the ’751 patent separate DNA fragments greater than or equal to 500 base pairs. The method steps of the ’931 patent separate DNA fragments greater than or equal to 300 base pairs. For example, claim 1 of the ’751 patent recites the fol- lowing method: 1. A method for preparing a deoxyribonucleic acid (DNA) fraction from a pregnant human female use- ful for analyzing a genetic locus involved in a fetal chromosomal aberration, comprising: (a) extracting DNA from a substantially cell-free sample of blood plasma or blood se- rum of a pregnant human female to obtain extracellular circulatory fetal and mater- nal DNA fragments; (b) producing a fraction of the DNA ex- tracted in (a) by: Case: 19-1419 Document: 55 Page: 19 Filed: 03/17/2020 4 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. (i) size discrimination of extracellu- lar circulatory DNA fragments, and (ii) selectively removing the DNA fragments greater than approxi- mately 500 base pairs, wherein the DNA fraction after (b) comprises a plu- rality of genetic loci of the extracellular circulatory fetal and maternal DNA; and (c) analyzing a genetic locus in the fraction of DNA produced in (b). Id. at col. 7 ll. 54–67, col. 8 ll. 53–57; cf., ’931 patent col. 7 ll. 58–67, col. 8 ll. 57–63 (claim 1). The dependent claims for each patent add detail such as techniques for conducting each method step and the de- tection of specific chromosomal aberrations. For example, claim 7 of the ’751 patent specifies centrifugation for the size discrimination step and claim 10 specifies for the de- tection of a fetal chromosomal aberration causing Down Syndrome. ’751 patent col. 9 ll. 1–2, 7–8. II. The Claims Are Not Patent Eligible The Majority sidesteps well-established precedent by reasoning that the claims in this case belong in a unique “bucket” reserved for patents that claim “a method of prep- aration.” 2 See Maj. Op. at 8. By placing this case in that bucket and not in a “diagnostic case” bucket, the Majority summarily dismisses precedent like Athena, Roche 2 Cf., Rapid Litig. Mgmt. Ltd. v. CellzDirect, Inc., 827 F.3d 1042, 1046 (Fed. Cir. 2016) (reciting in claim 1’s pre- amble “[a] method of producing a desired preparation”). Case: 19-1419 Document: 55 Page: 20 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 5 Molecular, Cleveland Clinic, Genetic Techs., Ariosa, 3 and others. Id. Our precedent, however, does not support the Majority’s per se grouping of claims. A “method of prepa- ration case” is treated no differently than any other process claim under our law. 35 U.S.C. § 101 grants patent rights to “[w]hoever in- vents or discovers any new and useful process[ 4], machine, manufacture, or composition of matter, or any new and useful improvement thereof.” See Ass’n for Molecular Pa- thology v. Myriad Genetics, Inc., 569 U.S. 576, 589 (2013). Laws of nature, natural phenomena, and abstract ideas, however, are not patent-eligible subject matter. Id. To determine whether a patent claims a patent-eligible application of a natural phenomenon or impermissibly mo- nopolizes a natural phenomenon, we apply the two-step test set forth by the Supreme Court. Alice Corp. v. CLS Bank Int’l, 573 U.S. 208, 217–18 (2014). In the first step, we determine whether the claims at issue are “directed to” a patent-ineligible concept. Id. If they are, we consider in the second step whether the additional claim elements— both individually and “as an ordered combination”—“trans- form the nature of the claim” into a patent-eligible applica- tion. Id. 3 Athena Diagnostics, Inc. v. Mayo Collaborative Servs., LLC, 915 F.3d 743 (Fed. Cir. 2019); Roche Molecu- lar Sys., Inc. v. Cepheid, 905 F.3d 1363 (Fed. Cir. 2018); Cleveland Clinic Found. v. True Health Diagnostics LLC, 859 F.3d 1352 (Fed. Cir. 2017); Genetic Techs. Ltd. v. Merial L.L.C., 818 F.3d 1369 (Fed. Cir. 2016); Ariosa Diag- nostics, Inc. v. Sequenom, Inc., 788 F.3d 1371 (Fed. Cir. 2015). 4 The term “process,” as recited in § 101, encom- passes all “process, art or method” claims. 35 U.S.C. § 100(b). Case: 19-1419 Document: 55 Page: 21 Filed: 03/17/2020 6 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. A. The Claims are Directed to a Patent-Ineligible Natural Phenomenon The claims are directed to a natural phenomenon be- cause the patent’s claimed advance is the discovery of that natural phenomenon. The Majority disregards well-estab- lished precedent for conducting the Alice, step one, “di- rected to” inquiry by failing to consider the patent’s claimed advance. The Supreme Court first articulated the “directed to” inquiry in Alice, 573 U.S. at 217–218. To make that deter- mination, the Court analyzed whether the claims “in- volved” patent-ineligible subject matter (there, an abstract idea). Id. at 218–220 (citing Gottschalk v. Benson, 409 U.S. 63, 71–72 (1972), and Bilski v. Kappos, 561 U.S. 593, 599 (2010)). In the three years following Alice, this court addressed numerous § 101 cases without articulating a more definite “directed to” inquiry. Instead, we performed step one of the patent-eligibility inquiry by comparing the claims at issue to the claims held eligible or ineligible in earlier Supreme Court and Federal Circuit cases. See, e.g., In re Smith, 815 F.3d 816, 818 (Fed. Cir. 2016); buySAFE, Inc. v. Google, Inc., 765 F.3d 1350, 1353 (Fed. Cir. 2014). Since 2016, in a string of cases reciting process claims, we began conducting the “directed to” inquiry by asking whether the “claimed advance” of the patent “improves upon a technological process or [is] merely an ineligible concept.” Athena, 915 F.3d at 750 (Lourie, J.); Genetic Techs., 818 F.3d at 1375. To determine a process’s “claimed advance,” we review the claims and the written description. Athena, 915 F.3d at 750. If a written description highlights the discovery of a natural phenomenon—e.g., by describing the natural phenomenon as the only “surprising” or “unexpected” as- pect of the invention or that the invention is “based on the Case: 19-1419 Document: 55 Page: 22 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 7 discovery” of a natural law—the natural phenomenon likely constitutes the claimed advance. See Ariosa, 788 F.3d at 1376; Athena, 915 F.3d at 751; Cleveland Clinic, 859 F.3d at 1360 –61. In Ariosa, we concluded that the claims were directed to a natural phenomenon based in part on the patent’s dis- closure that the natural phenomenon was a “surprising and unexpected finding.” 788 F.3d at 1376 (citation and quotation omitted). In Athena, we concluded that the claimed advance was “only in the discovery of a natural law” based in part on the patent’s disclosure that the in- ventors “surprisingly found” the natural law. 915 F.3d at 751 (citation and quotation omitted). In Cleveland Clinic, we concluded that the claims were directed to a natural law relying, in part, on the patent’s disclosure that “the inven- tions are ‘based on the discovery’” of the natural law. 859 F.3d at 1360–61 (citation omitted). Here, the claimed advance is the inventors’ “surpris- ing[]” discovery of a natural phenomenon—that cff-DNA tends to be shorter than cell-free maternal DNA in a mother’s bloodstream. See ’751 patent col. 1 ll. 54–61. Like in Ariosa and Athena, the patent’s written description iden- tifies the natural phenomenon as the only “surprising find- ing.” Id. at col. 1 l. 54–col. 2 l. 6. And the patent explains that the natural phenomenon “forms the basis of the pre- sent invention,” like the patent in Cleveland Clinic. Id. at col. 2 ll. 1–6. It is undisputed that the surprising discovery is a natural phenomenon. See Maj. Op. at 3–4, 8. The claimed advance is, therefore, the natural phenomenon. This conclusion is bolstered by the fact that the claimed method steps begin and end with a naturally occurring sub- stance, as in Ariosa. 788 F.3d at 1376. In Ariosa, we found ineligible process claims directed to a method of detecting paternally inherited cff-DNA. Id. The claimed method steps began with a naturally occurring blood sample and ended with cff-DNA, both naturally occurring substances. Case: 19-1419 Document: 55 Page: 23 Filed: 03/17/2020 8 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. Id. The inventors did not create or alter any of the genetic information encoded in the cff-DNA in the claimed method steps. Id. Likewise, the claimed method here begins and ends with a naturally occurring substance. The claimed method begins with extracting a sample of blood plasma or serum from a pregnant mother that consists wholly of various nat- urally occurring substances, including cff-DNA. ’751 pa- tent col. 7. ll. 58–61. The claimed method separates those naturally occurring substances by size, leaving a “fraction” of the original sample that is predominantly cff-DNA. Id. at col. 7 ll. 63–67, col. 8 ll. 53–55. The claimed method ends with analyzing the components of the “fraction,” which con- tains cff-DNA. Id. at col. 8 ll. 56–57. The substances pre- sent throughout the process are naturally occurring substances, and the claimed method steps do not alter those substances. The claimed method is therefore di- rected to a natural phenomenon. The Majority fails to identify the claimed advance The Majority’s step one analysis ignores the claimed advance inquiry altogether. Contrary to the Majority’s con- clusion, the claims here are not directed to “a patent-eligi- ble method that utilizes [the natural phenomenon].” Maj. Op. at 8–9. Although the Majority states that the claims “are directed to methods for preparing a fraction of cell-free DNA that is enriched in fetal DNA” (id. at 9), the Majority fails to address with specificity the patent’s claimed ad- vance. Instead, the Majority only seems to suggest that the claimed advance is an improvement in “size discrimi- nat[ion]” and “selective[] remov[al]” techniques. See id. at 9–10. The Majority reasons that the inventors used “spe- cific process steps” of “size discriminating and selectively removing DNA fragments that are above a specified size threshold” and that these “concrete process steps . . . exploit [the natural phenomenon] in a method for preparation of a Case: 19-1419 Document: 55 Page: 24 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 9 mixture enriched in fetal DNA.” Id. at 10–11. But whether the steps are concrete is not the appropriate analysis for determining the claimed advance. Where a written description identifies a technology as well-known or performed using commercially available tools or kits, that technology cannot logically constitute a claimed advance. Ariosa, 788 F.3d at 751; see also Athena, 915 F.3d at 751 (identifying the claimed “immunological assay techniques [as] known per se in the art” and there- fore not the claimed advance); Cleveland Clinic, 859 F.3d at 1361 (relying on the patent’s disclosure of “commercially available testing kits” for detecting the natural law). Here, the claimed advance is not an improvement in the underlying DNA-processing technology, as hinted by the Majority. The written description identifies the claimed method steps as well-known or performed using commercially available tools or kits. See ’751 patent col. 2 l. 49–col. 3 l. 18, col. 3 ll. 49–50, col. 3 l. 65–col. 4 l. 13, col. 5 ll. 45–50. For example, the table below highlights the commercially available tools and kits that are identified in the written description as used to perform each claimed method step. Case: 19-1419 Document: 55 Page: 25 Filed: 03/17/2020 10 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. Performance of Claimed Method Steps Commercially Available Claimed Method Step Tool or Kit QIAgen Maxi kit Claim 1(a), “extracting (’751 patent col. 3 ll. 49– DNA” 50) Invitrogen 1% agarose gel (’751 patent col. 3 ll. 66– 67) New England Biolabs 100 Claim 1(b)(i), “size dis- base pair ladder crimination” (id. at col. 4 ll. 4–5) Claim 1(b)(ii), “selec- Lamda Hind III digest tively removing” (’751 patent col. 4 ll. 5–6) QIAEX Gel Extraction kit (id. at col. 4 ll. 10–12) Applied Biosystems (ABI) 7000 Sequence Detection System (’751 patent col. 4 ll. 14– Step (c), “analyzing a ge- 38) netic locus” TaqMan System and TaqMan Minor Groove Binder (id. at col. 4 ll. 19–38) The selection of 300 and 500 base pairs resulted from using commercially available DNA size-markers. See id. at col. 4 ll. 3–9. The claimed DNA-processing technologies do not, therefore, constitute the claimed advance. See Cleveland Clinic, 859 F.3d at 1361. Case: 19-1419 Document: 55 Page: 26 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 11 The Majority relies on CellzDirect. See Maj. Op. at 12– 13. But CellzDirect is different from this case. In CellzDi- rect, the inventors created a new and useful cryopreserva- tion technique comprising multiple freeze-thaw cycles. 827 F.3d at 1048. The claimed invention went beyond applying a known laboratory technique to a newly discovered natu- ral phenomenon and, instead, created an entirely new la- boratory technique. Id. Unlike CellzDirect, the claimed method steps here are not new nor are the claimed tech- niques used in a new or unconventional way. The Majority recognizes that the inventors “did not invent centrifuga- tion, chromatography, electrophoresis, or nanotechnol- ogy”—the claimed techniques described in the written description. Maj. Op. at 13. The Majority’s remaining reasoning fails The Majority further reasons that the claimed method steps of size discrimination and selective removal “change the composition of the mixture, resulting in a DNA fraction that is different from the naturally-occurring fraction in the mother’s blood.” Id. at 10. On this basis, the Majority concludes that the claimed method in the patent “achieves more than simply observing that fetal DNA is shorter than maternal DNA, or detecting the presence of that phenome- non.” Id. The Majority’s reasoning is shortsighted. A process that merely changes the composition of a sample of natu- rally occurring substances, without altering the naturally occurring substances themselves, is not patent eligible. See Genetic Techs., 818 F.3d at 1374 (using PCR to amplify ge- nomic DNA in a sample before detecting it); Ariosa, 788 F.3d at 1373 (using PCR to amplify cff-DNA in a sample before detecting it). Here, the claimed method steps of size discrimination and selective removal do not alter the naturally occurring substances in the sample of blood plasma or serum from a pregnant mother. Cf., Myriad, 569 U.S. at 593 (“Myriad’s Case: 19-1419 Document: 55 Page: 27 Filed: 03/17/2020 12 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. claims are simply not expressed in terms of chemical com- position, nor do they rely in any way on the chemical changes that result from the isolation of a particular sec- tion of DNA.”). The Majority attempts to distinguish Myriad, reason- ing that the claims at issue in Myriad were not method claims. Maj. Op. at 12 (citing Myriad, 569 U.S. at 595). But I see no principled reason why, under the facts of this case, Myriad should or should not apply simply because this case presents a method claim and not a composition of matter claim. Whether the asserted claims recite a compo- sition of matter or a “method of preparation,” the purpose of § 101 remains the same, to safeguard against claims that monopolize a law of nature, natural phenomenon, or ab- stract idea. See Alice, 573 U.S. at 216 (“We have described the concern that drives this exclusionary principal as one of pre-emption.”). Because the patent’s claimed advance is the discovery of the natural phenomenon, the claims are directed to a natural phenomenon under the step one inquiry. B. The Claims Fail to Recite an Inventive Concept Step two of the Alice inquiry is a search for other ele- ments that transform the ineligible claims into signifi- cantly more than a patent upon the natural law or phenomenon. See Mayo Collaborative Servs. v. Prometheus Labs., Inc., 566 U.S. 66, 72–73. Mayo made clear that transformation into a patent eligible application requires “more than simply stat[ing] the law of nature while adding the words ‘apply it.’” Id. at 72. In step two, we ask: “[w]hat else is there in the claims before us?” Id. at 78. This question is a lifeline, one that is limited to “additional features” of the claim that trans- forms the nature of the claim into a patent-eligible appli- cation. Id. at 77; Ariosa, 788 F.3d at 1377. Case: 19-1419 Document: 55 Page: 28 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 13 For method claims that encompass natural phenom- ena, the method steps are the additional features that must be new and useful. See Parker v. Flook, 437 U.S. 584, 591 (1978) (“The process itself, not merely the mathematical al- gorithm, must be new and useful.”). We must assess whether the additional features are new and useful within the field generally, not in the context of their particular ap- plication to the newly discovered phenomenon. See Roche Molecular, 905 F.3d at 1372; see also Athena, 915 F.3d at 754. The method steps under review fail to transform the nature of the claims into patent-eligible applications. The three claimed method steps of (a) extracting DNA, (b) pro- ducing a fraction of DNA by size discrimination, and (c) an- alyzing a genetic locus are not new, either alone or in combination. The written description indicates that the la- boratory techniques of the claimed method are commer- cially available techniques. And the written description explains that step (b)’s producing a fraction by size discrim- ination “can be brought about by a variety of methods.” ’751 patent col. 2 ll. 49–51. For step two purposes, that the size discrimination and selective removal method steps were never before applied to the newly discovered natural phenomenon does not ren- der those steps new and useful. See Roche Molecular, 905 F.3d at 1372; see also Athena, 915 F.3d at 754. In Roche Molecular, we held that the method claims at issue, which involved PCR amplification of DNA, did not contain an in- ventive concept notwithstanding that the inventors were the first to use PCR to detect the claimed natural phenom- enon. Id. We reasoned that the claims did not contain an inventive concept because they did not “disclose any ‘new and useful’ improvement to PCR protocols or DNA amplifi- cation techniques in general.” Id.; see also Athena, 915 F.3d at 754 (noting that “to supply an inventive concept the sequence of claimed steps must do more than adapt a con- ventional assay to a newly discovered natural law”). Case: 19-1419 Document: 55 Page: 29 Filed: 03/17/2020 14 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. Like in Roche Molecular, the claimed method steps here do not disclose any new and useful improvement to DNA separation techniques. They do not disclose an un- conventional assay to the newly discovered natural phe- nomenon. Instead, they adapt commercially available DNA separation techniques to the natural phenomenon. The dependent claims also fail to transform the nature of the claims because they too rely on the same commer- cially available, routine, and conventional techniques as claim 1, only they provide more specificity on which tech- niques to use (e.g., ’751 patent, claim 7, identifies “density gradient centrifugation” for the claimed size discrimination method). Simply appending routine, conventional steps to a nat- ural phenomenon, specified at a high level of generality, is not enough to supply an inventive concept. Thus, under step two, the claims of the patent in this appeal that are directed to patent ineligible subject matter are not trans- formed and made eligible under Alice step two. III. Preemption The Supreme Court has made clear that the principle of preemption is the basis for the judicial exceptions to pa- tentability. Alice, 573 U.S. at 216–217. As Mayo empha- sized, “there is a danger that the grant of patents that tie up the[] use [of laws of nature] will inhibit future innova- tion premised upon them.” 566 U.S. at 86. Here, the claims are drafted in a manner that tie up future innovation premised upon the natural phenomenon because no skilled artisan would be entitled to rely on the natural phenomenon to isolate cff-DNA. That a skilled ar- tisan could isolate or enrich cff-DNA using some unclaimed technique is not dispositive for preemption. See Athena Di- agnostics, Inc. v. Mayo Collaborative Servs., 927 F.3d 1333, 1351 (Fed. Cir. 2019) (Chen, J., concurring with denial of the petition for rehearing en banc) (“That claims 7 and 9 do Case: 19-1419 Document: 55 Page: 30 Filed: 03/17/2020 ILLUMINA, INC. v. ARIOSA DIAGNOSTICS, INC. 15 not preempt all ways of observing the law of nature isn’t decisive, as none of the steps recited therein add anything inventive to the claims.”). Like in Athena, the only claimed advance here is the discovery of the natural phenomenon, and as drafted, these claims significantly preempt use of that natural phenomenon. I do not doubt that process claims that involve natu- rally occurring phenomena from beginning to end could be directed to patent eligible subject matter, but this is not such a case.